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KMID : 0545119950050010036
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 1 p.36 ~ p.40
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Valistain (3-Amino-2-Hydroxy 4-Phenylbutanoyl-Valyl-Valine), a New Aminopeptidase M Inhibitor, Produced by Streptomyces sp. SL20209
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Ko, Hack-Ryong
Chun, Hyo-Kon/Jung, Myung-Chul/Kho, Yong-Hee
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Abstract
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Valistatin, a new inhibitor of aminopeptidase M(AP-M) was discovered in the culture broth of Streptomyces sp. SL20209 isolated from a soil sample. The inhibitor was purified by extraction with n-butanol and the various column chromatographies, and then isolated as whitish powder. The ^1H-and ^1H, ^1H-COSY NMR studies, amino acid analysis, and fragmentation patterns by FAB-MS suggested the presence of one 3-amino-2-hydroxy-4-phenylbutanoic acid and two valine residues in the inhibitor. Thus, the structure of valistatin was determined as 3-amino-2-hydroxy-4-phenylbutanoyl-valyl-valine. Valistatin has the molecular formular C_20H_31N_3O_5, (MW 394), and its IC_50 value against hog kidney AP-M was determined to be 3.12㎍/㎖.
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